Table 1.
(A) Primary and (B) secondary antibodies
| Raised against | Host | Dilution | Source | Aimed at |
|---|---|---|---|---|
| (A) | ||||
| Human NSE | Rabbit | 1:10 | Calbiochem, San Diego, CA, USA | Neurons (cell body and processes) |
| Human HuD (L-20) | Goat | 1:200 | Santa Cruz Biotechnology, Santa Cruz, CA, USA | Neurons (cell body) |
| nNOS | Rabbit | 1:200 | Calbiochem | Nitrergic neurons |
| ChAT | Rabbit | 1:1000 | Kind gift from Dr. Schemann, Hannover, Germany | Cholinergic neurons |
| Cleaved caspase-3 | Rabbit | 1:100 | Cell Signaling Technology, Danvers, MA, USA | Apoptotic cells |
| Bcl-2 | Rabbit | 1:1500 | Chemicon International Inc., Temecula, CA, USA | Antiapoptotic protein Bcl-2 |
| VIP | Rabbit | 1:250 | Chemicon International | VIPergic neurons |
| SP | Rabbit | 1:250 | Chemicon International | SPergic neurons |
| Human c-kit (CD117) | Rabbit | 1:100 | DakoCytomation, Glostrup, Denmark | Interstitial cells of Cajal |
| Bovine GFAP | Rabbit | 1:250 | DakoCytomation | Enteric glial cells |
| Antibody | Host | Dilution | Source | Used against |
|---|---|---|---|---|
| (B) | ||||
| Anti-rabbit IgG*TRITC | Goat | 1:600 | Sigma Chemicals, Saint Louis, MO, USA | NSE |
| Anti-goat IgG*Alexa Fluor 555 | Donkey | 1:1000 | Molecular Probes, Inc, Eugene, OR, USA | HuD |
| Anti-rabbit IgG*Alexa Fluor 488 | Donkey | 1:1500 | Molecular Probes | nNOS, ChAT, Bcl-2, cleaved caspase-3 |
| Anti-rabbit IgG*CY3 | Goat | 1:150 | Amersham Int., Arlington Heights, IL, USA | VIP, SP, GFAP, c-kit |
GFAP, glial fibrillary acidic protein; NSE, neuron-specific enolase.