(A) |
Human NSE |
Rabbit |
1:10 |
Calbiochem, San Diego, CA, USA |
Neurons (cell body and processes) |
Human HuD (L-20) |
Goat |
1:200 |
Santa Cruz Biotechnology, Santa Cruz, CA, USA |
Neurons (cell body) |
nNOS |
Rabbit |
1:200 |
Calbiochem |
Nitrergic neurons |
ChAT |
Rabbit |
1:1000 |
Kind gift from Dr. Schemann, Hannover, Germany |
Cholinergic neurons |
Cleaved caspase-3 |
Rabbit |
1:100 |
Cell Signaling Technology, Danvers, MA, USA |
Apoptotic cells |
Bcl-2 |
Rabbit |
1:1500 |
Chemicon International Inc., Temecula, CA, USA |
Antiapoptotic protein Bcl-2 |
VIP |
Rabbit |
1:250 |
Chemicon International |
VIPergic neurons |
SP |
Rabbit |
1:250 |
Chemicon International |
SPergic neurons |
Human c-kit (CD117) |
Rabbit |
1:100 |
DakoCytomation, Glostrup, Denmark |
Interstitial cells of Cajal |
Bovine GFAP |
Rabbit |
1:250 |
DakoCytomation |
Enteric glial cells |