Table 2.
Microscopic measurements of the mucosal and muscle layers of the ileum and mid-colon of control and diabetic mice
| Ileum | Mid-Colon | ||||
|---|---|---|---|---|---|
| Control | Diabetic | Control | Diabetic | ||
| Mucosal layer | |||||
| No. villi/mm | 12.4 ± 0.5 | 12.0 ± 0.7 | No. crypts/mm | 23.4 ± 1.8 | 22.0 ± 1.3 |
| Villus length (μm) | 184.8 ± 11.0 | 224.6 ± 12.0* | |||
| Villus width ((μm) | 83.7 ± 3.9 | 73.1 ± 3.8** | Crypt width (μm) | 39.6 ± 1.4 | 43.8 ± 1.1* |
| Crypt depth (μm) | 105.6 ± 7.0 | 116.2 ± 16.0 | Crypt depth (μm) | 288.7 ± 13.0 | 271.0 ± 10.6 |
| Muscle layer (thickness) | |||||
| Circular (μm) | 25.3 ± 2.2 | 30.5 ± 2.9* | Circular (μm) | 114.7 ± 9.5 | 84.5 ± 5.3* |
| Longitudinal (μm) | 15.5 ± 1.3 | 16.8 ± 1.2 | Longitudinal ((μm) | 22.2 ± 2.9 | 23.2 ± 1.7 |
| Whole-muscle layer (μm) | 40.8 ± 3.5 | 47.2 ± 4.1* | Whole-muscle layer (μm) | 136.7 ± 7.6 | 107.4 ± 4.9* |
Figures represent mean ± SEM. The mean value for each animal was obtained from 10 and 8 observations in the mucosal and muscle layer respectively. Significance was tested with the Student's t-test.
*
P<0.05 vs. control mice
**
P<0.01 vs. control mice.