Table 2.

Body weight and plasma parameters in db/db and ob/ob mice after 1 and 3 months of standard chow or high-calorie (HC) diet

	Standard chow		HC diet
	1 month	3 months	1 month	3 months
db/db mice	n = 9	n = 10	n = 10	n = 9
Body weight (g)	44.5 ± 2.3	52.3 ± 1.0*	53.1 ± 0.9†	58.6 ± 1.9*†
Leptin (ng/ml)	144 ± 18	215 ± 15	208 ± 27	179 ± 35
β-Hydroxybutyrate (mM)	0.22 ± 0.05	0.20 ± 0.03	0.14 ± 0.03	1.90 ± 0.97*†
NEFAs (mM)	1.58 ± 0.43	1.62 ± 0.66	1.29 ± 0.07	1.49 ± 0.21
Triglycerides (mM)	2.17 ± 0.16	2.32 ± 0.40	1.89 ± 0.12	2.00 ± 0.26
Total cholesterol (mM)	3.49 ± 0.28	4.33 ± 0.24	7.80 ± 0.42†	8.31 ± 1.17†
Glucose (mM)	58.2 ± 4.7	64.5 ± 8.2	60.0 ± 4.9	57.0 ± 4.3
Antioxidant status (mM)	1.16 ± 0.08	1.36 ± 0.09	1.33 ± 0.08	1.34 ± 0.13
ALT (UI/l)	660 ± 229	506 ± 158	979 ± 335	794 ± 369
LDH (UI/l)	8234 ± 2468	4400 ± 672	7440 ± 2526	5643 ± 2075
ob/ob mice	n = 10	n = 10	n = 10	n = 10
Body weight (g)	47.5 ± 1.3	56.2 ± 1.0*	51.1 ± 1.4†	59.2 ± 1.0*†
β-Hydroxybutyrate (mM)	0.08 ± 0.02	0.07 ± 0.01	0.07 ± 0.02	0.07 ± 0.01
NEFAs (mM)	0.92 ± 0.09	0.97 ± 0.09	1.37 ± 0.13†	0.99 ± 0.09*
Triglycerides (mM)	1.10 ± 0.10	1.22 ± 0.09	1.42 ± 0.20	1.21 ± 0.14
Total cholesterol (mM)	5.02 ± 0.14	5.24 ± 0.53	6.26 ± 0.39†	5.91 ± 0.34†
Glucose (mM)	23.0 ± 2.9	20.3 ± 1.8	23.3 ± 4.0	21.3 ± 2.0
Antioxidant status (mM)	1.16 ± 0.05	1.20 ± 0.05	1.04 ± 0.07	1.30 ± 0.09*
ALT (UI/l)	681 ± 225	592 ± 118	218 ± 39	400 ± 212
LDH (UI/l)	6982 ± 3070	5217 ± 772	5386 ± 812	3827 ± 1094

Blood was drawn in the fed state. Results are means ± SEM for the number of mice specified in the table, excepted for leptin which was assessed in 4–8 db/db mice per group.

Symbols indicate statistical significance between groups

^*Different from 1 month (P<0.05).

^†Different from standard chow (P<0.05).

ALT, alanine aminotransferase; LDH, lactate dehydrogenase; NEFA, non-esterified fatty acids.