Table 3.
Pathological features of liver lesions in db/db and ob/ob after 1 and 3 months of standard chow or high-calorie (HC) diet
| Standard chow | HC diet | |||||||
|---|---|---|---|---|---|---|---|---|
| ob/ob | db/db | ob/ob | db/db | |||||
| 1 month | 3 months | 1 month | 3 months | 1 month | 3 months | 1 month | 3 months | |
| Steatosis | ||||||||
| Percentage | 82 ± 1 | 80 ± 3 | 58 ± 8† | 69 ± 3† | 92 ± 2§ | 89 ± 2§ | 86 ± 2†§ | 76 ± 2†§* |
| Type | ||||||||
| Micro | ++ | + | +++ | ++ | ++ | + | +++ | ++ |
| Medio | ++ | ++ | + | + | ++ | ++ | + | + |
| Macro | + | ++ | + | ++ | + | ++ | + | ++ |
| Necroinflammation | ||||||||
| 0 | 1 | 1 | 4 | 0 | 0 | 0 | 0 | 2 |
| 1 | 8 | 7 | 5 | 8 | 9 | 10 | 8 | 4 |
| 2 | 1 | 2 | 0 | 2 | 1 | 0 | 2 | 3 |
| Apoptosis Percentage | 3 ± 1 | 0 ± 0* | 13 ± 2† | 15 ± 1† | 10 ± 1§ | 7 ± 1§ | 15 ± 2 | 11 ± 1 |
| Portal fibrosis | ||||||||
| 0 | 6 | 0 | 5 | 1 | 1 | 1 | 1 | 0 |
| 1 | 4 | 10 | 4 | 9 | 9 | 9 | 9 | 7 |
| 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
| Perisinusoidal fibrosis | ||||||||
| 0 | 10 | 5 | 9 | 6 | 8 | 4 | 10 | 2 |
| 1 | 0 | 4 | 0 | 4 | 2 | 5 | 0 | 6 |
| 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 |
| NAS | ||||||||
| 0–2 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 0 |
| 3–4 | 9 | 8 | 4 | 8 | 9 | 10 | 8 | 6 |
| 5–8 | 1 | 2 | 0 | 2 | 1 | 0 | 2 | 3 |
Results for steatosis and apoptosis are means ± SEM for 9–10 mice. Results for necroinflammation and fibrosis are given as the number of mice presenting a score of 0, 1 or 2 respectively. Results for NAS (non-alcoholic fatty liver disease activity score) are given as the number of mice presenting scores of 0–2, 3–4 or 5–8 respectively.
Symbols indicate statistical significance between groups
*
Different from 1 month (P<0.05).
†
Different from ob/ob mice of the same age (P<0.05).
§
Different from standard chow (P<0.05).