Fig. 2.
Invariant natural killer T (iNKT) cells are recruited to allogeneic but not syngeneic skin and heart grafts in multiple mouse strain combinations. CBA (syngeneic) or C57BL/10 (allogeneic) skin grafts were analysed by quantitative real-time polymerase chain reaction (qRT–PCR) for Vα14Jα18 mRNA expression (to quantify the infiltration of iNKT cells) on days 3, 5 and 10 post-transplant (a). In addition, heart grafts taken from B6 (H2b) recipients of either syngeneic (C57BL/6) or allogeneic (BALB/c) grafts were analysed for the expression of Vα14Jα18 mRNA on days 1, 3 and 5 post-transplant (b). Results are expressed as mRNA [units/hypoxanthine guanine phosphoribosyl transferase (HPRT)] with naive skin or heart used to give baseline expression. n = 3 per time-point. All error bars indicate mean ±standard deviation.*P ≤ 0·05.