Fig. 3.

Detection of the percentages of the DR^low+CD45RA^-, DR^high+CD45RA^-, DR^-CD45RA^- and DR^-CD45RA⁺ T_reg subsets within the total CD4⁺CD127^low+/−CD25⁺forkhead box protein 3 (FoxP3⁺) T_reg cell pool during the normal course of pregnancy. The figure shows the dot-blots of the gated DR^low+CD45RA^-, DR^high+CD45RA^-, DR^-CD45RA^- and DR^-CD45RA⁺ T_reg subsets and their quantitative changes during the normal course of pregnancy. The diagram presents the individual and median data obtained for non-pregnant (NP) and healthy pregnant women. The percentage of the DR^low+CD45RA^- T_reg subset (a,b) and of the DR^high+CD45RA^- T_reg subset (c,d) decreased significantly during the 10th and 20th weeks of gestation. In contrast, the percentage of the DR^-CD45RA⁺ T_reg subset increased during the same time (e,f). The percentage of the DR^-CD45RA^- T_reg subset did not show any significant changes during the whole course of pregnancy (g,h).