Fig. 4.

Detection of the percentages of the DR^low+CD45RA^-, DR^high+CD45RA^-, DR^-CD45RA^- and DR^-CD45RA⁺ T_reg subsets within the total CD4⁺CD127^low+/−CD25⁺forkhead box protein 3 (FoxP3⁺) T_reg cell pool in healthy and complicated pregnancies. The figure presents the individual data (a–h) and median values (i–l) obtained for pregnancies affected by pre-eclampsia () or HELLP syndrome () (a–d), and for pregnancies affected by CI () or PL () (e–h), in comparison to healthy pregnancies (HP) (♦). Pregnancies affected by pre-eclampsia revealed significantly increased percentages of the DR^low+CD45RA^- and DR^high+CD45RA^- T_reg subsets (a,b,i,j), while in pregnancies affected by HELLP syndrome only the DR^high+CD45RA^- T_reg subset was increased significantly (b,j). In contrast, the DR^-CD45RA^- and DR^low+CD45RA^- T_reg subsets were increased significantly in pregnancies affected by PL (e,g,i,k). The percentage of the naive DR^-CD45RA⁺ T_reg subset was decreased significantly in pregnancies affected by both pre-eclampsia and PL (d,h,l). CI: cervical insufficiency; PL: preterm labour necessitating preterm delivery; HELLP syndrome: haemolysis-elevated liver enzyme levels-low platelet count syndrome; MFI: mean fluorescence intensity.