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. 2012 Jan;167(1):99–107. doi: 10.1111/j.1365-2249.2011.04494.x

Fig. 1.

Fig. 1

Expression of Nck1 protein was reduced after Nck1-specific short interfering (si)RNA transfection. E6-1 Jurkat T cells (a) and primary CD4 T cells (b) were transfected with Nck1-specific or negative control siRNA for 48 h. Cell extracts were subjected to Western blot analysis with anti-Nck1 monoclonal antibody (mAb) and anti-β-actin mAb. (a) Lane 1, blank control; lane 2, transfection with 100 pmol Nck1 siRNA; lane 3, transfection with 50 pmol Nck1 siRNA; lane 4, transfection with 100 pmol non-specific siRNA negative control; lane 5, transfection with 50 pmol non-specific siRNA negative control. (b) Lane 1, untransfected CD4 T cells; lane 2, negative control siRNA; lane 3, si RNA Nck1.