Fig. 2.

Nck1 knock-down did not induce apoptosis in Jurkat T cells. E6-1 Jurkat T cells were transfected with Nck1-specific short interfering (si)RNA (a), untransfected (b) or transfected with non-specific siRNA negative control (c). Jurkat T cells were also stimulated with anti-T cell receptor (TCR) monoclonal antibody (mAb) and there was no difference in the % apoptosis of T cell receptor (TCR)-stimulated Jurkat cells transfected with Nck1-specific siRNA (d), untransfected (e) or transfected with non-specific siRNA negative control (f). The lower left zone showed the viable cells (annexinV^-/PI^-), the upper left zone represented necrosis cells (annexinV^-/PI⁺), the lower right zone showed early apoptosis (annexinV⁺/PI^-) and the upper right zone showed the late apoptosis (annexinV⁺/PI⁺). The combination of the upper and lower left zones represented the total apoptotic cells. Shown are mean percentage ± standard deviation of apoptotic cells from three experiments.