Fig. 1.

NH₂ truncation of recombinant CXCL10 by dipeptidylpeptidase IV (DPP4). Human recombinant CXCL10 (1–77) was incubated in vitro with recombinant DPP4 to obtain CXCL10 (3–77). (a) Native or digested protein (150 ng) was loaded onto a 15% bis-acrylamide gel for Western blot analysis; (b) 0·5 µg was spotted on an H4 column for mass spectrometry analysis. The difference in molecular weight between CXCL10 (1–77) and CXCL10 (3–77) is consistent with a two amino acid truncation.