Fig. 1.
Collagen-V (Col-V) antibody characterization for individual fragments α1(V) and α2(V) by Western blot analysis on bronchiolitis obliterans syndrome [BOS(+)] patients. (a) Western blot analysis of membrane strips coated by α1(V) and α2(V) individually. Patients 1–12 refer to the 12 BOS(+) lung transplant (LTx) recipients, ‘N’ refers to a representative of two (of five tested) normal healthy adults, and ‘BN’ refers to the representive of two (of seven) time-matched stable LTx recipients. (B) Serial analysis for the development of antibodies to α1(V) and α2(V) from 2 years before onset of BOS to 18 months after the onset of BOS; and (c) concentrations of antibodies to whole Col-V, α1(V) and α2(V). The presented numbers of mean ± standard error of the mean for 12 patients.