Figure 3: Effect of RNAi-mediated knockdown of p300 on GFAP and Nestin expression.

(A & B) Five p300-shRNA stable clones (Sh#1-clones: 10, 20 and 27; Sh#3-clones: 10 and 12), one vector control and parental U251 cells (1×10⁶) were transfected with 2 µg of GFAP-Luc (A), and 2 µg of Nestin-Luc (B). Luciferase activity was measured at 72 h posttransfection and normalized percent luciferase activities were plotted as mean ± SE (n=3) (* and ** indicate p < 0.05 and p < 0.01 respectively). U251 stable clones overexpressing p300 shRNA (Sh#1-clone 10 and Sh#3-clone 10) were stained for GFAP (green) (C) and Nestin (red) (D) using specific antibodies.