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. 2010 Aug 4;1(4):278–288. doi: 10.18632/oncotarget.138

Figure 2: MYCN binds to the B-MYB promoter in vivo and induces histones acetylation.

Figure 2:

A) distribution of the five putative E-BOXes on the B-MYB promoter found by the TESS analyzer tool [www.cbil.upenn.edu/cgi-bin/tess/tess]. B) Chromatin IP analysis of the MYCN protein bound onto the B-MYB promoter in SHSY5Y and SKNAS (non MYCN amplified) and LAN-1(MYCN amplified) neuro-blastoma cell lines. Cross-linked chromatin was immuno-precipitated with MYCN or GAL4, used as a negative control, antibodies as indicated. C) Chromatin-IP analysis to assess the presence of acetylated histones H3 and H4 on the B-MYB promoter region containing the putative MYCN binding sites. The antibodies used are indicated on the top of the figure. D) Chromatin-IP analysis of factors bound onto the B-MYB promoter in the presence or absence of inducible MYCN. Antibodies used and conditions are shown in the top of the panel.