Skip to main content
. 2010 Aug 4;1(4):278–288. doi: 10.18632/oncotarget.138

Figure 5: B-MYB binds to and regulates the MYCN promoter.

Figure 5:

A) Chromatin-IP analysis of proteins bound onto the MYCN promoter region encompassing the putative MYB-binding sites. Antibodies to immunoprecipitate B-MYB, acetylated histones H3 and H4 or GAL4 (used as a negative control) in LAN-1 [MYCN amplified] cells were used, as indicated in the top of the panel. B) Luciferase assay to assess MYCN promoter (MYCNp-pGL2) transcriptional activity in the presence or absence of exogenously expressed B-MYB. This assay was repeated several times until it reached statistical significance [*p < 0.01] C] Western blot analysis of IMR32 cell lines infected with lentiviruses expressing, in order from left to right, sh::scrambled, sh::B-MYB and sh::MYCN, and selected with puromycin for 6 days. Whole cell lysates were prepared and subjected to western blot analysis with the indicated antibodies. Relative intensity of the bands are indicated on the top of the lanes.