Figure 6. Analytical Superose6 size-exclusion chromatography.

(A) Size-exclusion chromatogram highlighting the separation of the Hsp90₂-FKBP52₁-HOP₂ complex (peak 1) from unbound constituents (peaks 2 and 3). (B) 10% SDS-PAGE results of the three respective chromatographic peaks. (C) A linear regression of the Superose6 elution positions of protein standards was used to interpolate the molecular weights of the proteins eluting within the three peaks. The solid square and triangles represent the interpolated molecular weight values of the Hsp90₂-FKBP52₁-HOP₂ complex and unbound constituents derived from the linear regression (R2=0.98). The hollow square denotes the molecular weight value of the Hsp90₂-FKBP52₁-HOP₂ complex derived from primary sequence information