Skip to main content
. 2011 Aug 23;2(8):599–609. doi: 10.18632/oncotarget.311

Figure 5. Combined exposure to EGFR antibody and Aurora kinase inhibitor results in fortified growth inhibition and apoptosis.

Figure 5

(A) SCCHN cells were treated for a total of 14 days with cetuximab (200 nM), the Aurora kinase inhibitor R763, the combination of both (Cet+ R763), or carrier only (control). The cell number was counted at the indicated times and the -fold increase in cell number calculated. Note that the increase in cell number is given in a logarythmic scale. The combination of cetuximab and Aurora kinase inhibitor resulted in a significantly reduced -fold increase after 14 day treatment period in all cell lines investigated in comparison to all other conditions (Cet alone, R763 alone, control; p<0.05). (B) The indicated SCCHN cells were cultured for a 48 hr period with the indicated conditions (cetuximab 200 nM, R7635 nM) and assessed for DNA content by PI staining. The percentage of polyploid cells with a DNA content >4n is given. (C) Analysis of the cells shown in (B) for apoptosis (Annexin V-positive, PI-negative cell fraction) by flow cytometry. The bars represent the mean ± SD of 3 independently performed experiments. Statistically significant differences are marked (* indicates p<0.05).