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. Author manuscript; available in PMC: 2012 Aug 10.
Published in final edited form as: Cell Immunol. 2011 Aug 10;271(2):342–349. doi: 10.1016/j.cellimm.2011.07.014

Figure 6.

Figure 6

Regulation of ISRE promoter activity by IRF. IRF3, IRF5, IRF7, or sc siRNA-treated THP-1 cells were transfected with an IRSE luciferase reporter construct. Cells were stimulated with poly (I-C) (10 μg/mL) for 6 h, and THP1 lysates were assayed for luciferase activity normalized to Renilla reniformis luciferase. Cells transfected with sc siRNA were used as control. A. ISRE promoter activity was decreased to baseline by IRF3 knockdown (p < 0.001; n = 3). There was also a significant reduction of approximately 50% in ISRE promoter activation with IRF7 and IRF5 deficiency (p < 0.001). B. IRF3/5/7 deficiency had no effect on AP-1 promoter activity (n = 3). C. IRF3/5/7 did not contribute to NF-κB promoter activation as determined by luciferase activity. D. IRF7 deficiency did not regulate IL-6 promoter activation as measured by relative luciferase activity.