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. 2011 Dec 27;122(1):142–152. doi: 10.1172/JCI43134

Figure 7. HFD feeding inhibits remodeling of the ARN energy-balance circuit.

Figure 7

ARN neurons were labeled with BrdU during embryogenesis. Mice were divided into a HFD-fed and a chow-fed cohort at 6 weeks, and BrdU-labeled embryonic neurons were assayed after 10 weeks of differential feeding. (A–C) In mice continually fed a chow diet, few labeled ARN neurons (BrdU [red] and DAPI [blue]) remained at 16 weeks, but a significantly greater number of labeled ARN neurons remained in littermates placed on HFD at 6 weeks of age. (D–F) A similar retention of labeled NPY neurons (yellow arrowheads) was found in HFD-fed mice (BrdU [red] and NPY [green]). (G–I) A similar retention of labeled POMC neurons (yellow arrowheads) was found in HFD-fed mice (BrdU [red] and POMC [green]). (J) Schematic of analysis. Mean ± SEM. Scale bar: 100 μm.