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. 2011 Dec 19;122(1):178–191. doi: 10.1172/JCI58128

Figure 5. Pro-tumorigenic activity of EETs is mediated by VEGF production in the tumor stroma and loss of sEH.

Figure 5

(A) LLC tumors in VEGF-LacZ-Tr mice treated with 14,15-EET (15 μg/kg/d) show β-galactosidase staining (marker of VEGF production) in tumor endothelium and stromal fibroblasts (arrows). Scale bars: 20 μm. (B) Expression of sEH, but not CYP2J and CYP2C, is downregulated in tumor (TEC) versus normal (NEC) ECs (two left panels). Control tissue: mouse liver. Expression of sEH, but not CYP2J and CYP2C, is downregulated in tumor lysates from larger LLC tumors (>5 cm3) versus smaller LLC tumors (<1 cm3) (third panel). sEH expression (brown staining) is also downregulated in B16F10 melanoma liver metastasis compared with normal adjacent liver (far right panel). Scale bar: 100 μm.