Figure 3. APNG loss confers sensitivity to primary GBM6 cells.

(A) Western blot showing protein expression of pooled shRNA stables. KD, knockdown. (B) Cell viability of GBM6 cells expressing pooled stable shRNA of APNG, MGMT, or both. (C) Cleaved caspase assay of GBM6 cells with knockdown of APNG, MGMT, or both when exposed to TMZ. (D) Comet tail assay measuring DNA damage of GBM6 cells with knockdown of APNG, MGMT, or both when exposed to 100 μm TMZ. Tail lengths are denoted. Knockdown of either APNG or MGMT increased tail length; knockdown of both enzymes produced a synergistic effect. (E) Representative images of comet tails quantified in D. *P < 0.05, ***P < 0.001.