Figure 5. Role of APNG and MGMT in GBM xenograft models.

(A) Representative IHC images of APNG-positive and -negative GBM explants. Scale bar: 50 μm. (B) Xenograft GBM explants were subjected to various modalities of treatment and compared within each treatment with respect to median overall survival, based on APNG IHC expression. (C) Xenograft GBM explants were subjected to TMZ and RT and compared within each treatment with respect to median overall survival, based on combined APNG IHC expression and MGMT promoter methylation. (D) Overall survival of A172 cells expressing EV, APNG, MGMT, or both APNG and MGMT injected into orthotopic (intracranial) xenograft models treated with TMZ. n = 7 (TMZ); 5 (untreated). (E and F) IHC quantification of Ki67 (E) and TUNEL (F) staining. 10 low-magnification fields per mouse were used, and 4 mice total per group assessed. Mean and SEM are shown. Data are mean and SEM. *P < 0.05, **P < 0.01, ***P < 0.001.