Figure 9. In large clinical trial datasets, APNG expression predicts poorer overall survival.

Patient samples were analyzed on TMAs by IHC for APNG expression. (A) There was no statistical difference in overall survival with respect to APNG expression in a GBM patient cohort treated with surgery and RT only (pre-TMZ era). (B) In patients treated within the EORTC-NCIC clinical trial, there was a statistically significant decrease in overall survival in patients whose tumor exhibited nuclear APNG. (C) In the EORTC-NCIC subgroup containing patients with a methylated MGMT promoter, APNG-positive patients had poorer survival than did their APNG-negative counterparts, but the difference was not significant. (D) In the EORTC-NCIC subgroup with an unmethylated MGMT, there was a significant decrease in overall survival in APNG-positive patients.