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. 2011 Dec 27;122(1):153–162. doi: 10.1172/JCI59660

Figure 2. Effect of HFD feeding on hypothalamic microglial markers in rats.

Figure 2

(A) Time course of hypothalamic microglia-specific (Cd68 and Emr1) and astrocyte-specific (Gfap) gene expression in rats fed chow or HFD for up to 28 days (n = 6/group). *P < 0.05 versus chow. (B) Correlation of hypothalamic Emr1 mRNA level (linearized using difference in CT count between the Gapdh and the Emr1 gene [ΔCT]) with change in fat mass (g) over 4 weeks of HFD feeding (r = 0.70; P < 0.001).