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. 2011 Dec 27;122(1):153–162. doi: 10.1172/JCI59660

Figure 4. Time course of the effect of HFD feeding on hypothalamic astrocytes.

Figure 4

(A–D) Representative images of astrocytes identified by immunohistochemical detection of GFAP protein in coronal sections of hypothalamus (10 μm) obtained from 10-week-old mice fed either (A) chow or HFD for (B) 1 week, (C) 2 weeks, or (D) 3 weeks. (E and F) GFAP staining of hypothalamic sections from 8-month-old mice fed (E) chow or (F) HFD. Scale bar: 50 μm. (G) Quantification of GFAP staining intensity (mean ± SEM) in the region of the ARC from mice fed either chow or HFD for 1 to 3 weeks (n = 6/group). (H) Quantification of GFAP staining intensity (mean ± SEM) in mice fed chow or HFD for 8 months. *P < 0.05 versus chow. The dashed boxes indicate the region used for quantification in G and H.