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. 2011 Nov 22;26(1):37–51. doi: 10.1210/me.2011-1109

Fig. 5.

Fig. 5.

Knockdown via siRNAs decreases the 1,25-(OH)2D3-mediated effect on c-MYC transcription. A, siRNA for Lamin A (LMNA, control), VDR, TCF4, or c-FOS (50 nm) were transfected into LS180 cells and 48 h later treated for an additional 24 h with ethanol vehicle (gray) or 1,25-(OH)2D3 (10−7m, black) in triplicate. RNA was reverse transcribed and analyzed by qPCR for c-FOS, c-MYC, VDR, and TCF4. *, P < 0.05 as compared with vehicle treatment within same siRNA. a, P < 0.05 as compared with LMNA control levels within the same treatment. These results are representative of at least three similar experiments. B, An independent experiment was performed to determine the basal contributions of β-catenin to each gene. siRNA for mock (no RNA control), Lamin A (LMNA, control), VDR, or β-catenin (50 nm) were transfected into LS180 cells and 48 h later treated for an additional 24 h with ethanol vehicle (gray) in triplicate. RNA was reverse transcribed and analyzed by qPCR for VDR, c-MYC, and β-catenin expression. a, P < 0.05 as compared with LMNA control levels within the same treatment. These results are representative of at least three similar experiments.