Fig. 2.

Multiple elements in GnRH-P are involved in AR-mediated repression. A, Known transcription factor binding sites in GnRH-P. GT1-7 cells were transiently transfected with the AR expression plasmid and reporter plasmids containing RSVe and GnRH-P with block deletions of the bases between −127 and −75 (Δ127/75) or between −70 and −28 (Δ70/28) (B) or GnRH-P or heterologous reporters containing the indicated promoter region between RSVe and RSVp (C). Cells were treated for 24 h with 100 nm R1881 (closed bars) or ethanol vehicle (open bars) and subjected to luciferase assay. Data are expressed relative to vehicle-treated GnRH-P. *, P < 0.05; and ***, P < 0.001 vs. vehicle by one-way ANOVA followed by Student's t test.