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FXR is expressed in the mouse lung. A, RT-QPCR analysis of expression levels of FXR transcript in WT mouse ileum, WT lung, and FXR−/− lung. B, Immunoblot analysis of FXR protein levels in WT and FXR−/− lung tissues. C, QRT-PCR analysis of expression levels of FXR transcript from isolated lung EC. D, Immunofluorescence staining of FXR and Pecam-1 on frozen lung tissues sections (green, FXR; red, Pecam-1; blue, DAPI). **, P < 0.01.
