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. 2011 Nov 10;26(1):87–94. doi: 10.1210/me.2011-1168

Fig. 3.

Fig. 3.

BMP3 suppresses BMP-Smad signaling. A, W20-17 cells were cotransfected with empty vector or BMP3 along with BMP4. At 24 h after transfection, protein levels were determined by Western blot. The protein levels were quantified by using Image J software. Smad1 was used for protein normalization. BMP3 reduced the amount of phospho- (P-)Smad1/5/8 induced by BMP4. B and C, W20-17 cells were cotransfected with 0, 30, 60, or 90 ng BMP3 expression vector or 0, 30, 60, or 90 ng empty vector along with 45 ng IdWT4F-luc reporter and 15 ng phRL-SV40 internal control vector. Luciferase activity was then stimulated by transfection of 50 ng BMP4 expression vector (B) or 50 ng BMP2 expression vector (C). BMP3 was able to reduce the luciferase activity induced by both BMP4 and BMP2. Data are shown as the mean ± sd (n = 3). **, P < 0.01 in comparison with cells transfected with an empty vector and BMP4 or BMP2 in each group.