Table 2. Glycosylation prediction servers.

Server	Portable Version	Method	Description	URL
NetNGlyc	Only for academics	ANN	N-glycosylation	http://www.cbs.dtu.dk/services/NetNGlyc/
EnsembleGly	No	SVM	N-glycosylation	http://turing.cs.iastate.edu/EnsembleGly/
EnsembleGly	No	SVM	O-glycosylation	http://turing.cs.iastate.edu/EnsembleGly/
EnsembleGly	No	SVM	C-glycosylation	http://turing.cs.iastate.edu/EnsembleGly/
GPP	No	RFM	N-glycosylation	http://comp.chem.nottingham.ac.uk/glyco/
GPP	No	RFM	O-glycosylation	http://comp.chem.nottingham.ac.uk/glyco/
NetOGlyc	Only for academics	ANN	O-glycosylation	http://www.cbs.dtu.dk/services/NetOGlyc/
Oglyc	No	SVM	O-glycosylation	http://www.biosino.org/Oglyc/
CKSAAP_OGlySite	No	SVM	O-glycosylation	http://bioinformatics.cau.edu.cn/zzd_lab/CKSAAP_OGlySite/
YinOYang	Only for academics	ANN	O-glycosylation	http://www.cbs.dtu.dk/services/YinOYang/
Big-PI	No		GPI anchor	http://mendel.imp.ac.at/gpi/gpi_server.html
GPI-SOM	Yes	ANN	GPI anchor	http://gpi.unibe.ch/
FragAnchor	No	ANN and HMM	GPI anchor	http://navet.ics.hawaii.edu/~fraganchor/NNHMM/NNHMM.html
PredGPI	Only for academics upon request	HMM and SVM	GPI anchor	http://gpcr.biocomp.unibo.it/predgpi/
NetCGlyc	Only for academics	ANN	C-mannosylation	http://www.cbs.dtu.dk/services/NetCGlyc/

ANN, artificial neural network; SVM, support vector machine; RFM, random forest method; HMM, hidden Markov model.