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. Author manuscript; available in PMC: 2011 Dec 29.
Published in final edited form as: J Immunol. 2010 Dec 27;186(3):1656–1665. doi: 10.4049/jimmunol.1002093

FIGURE 2.

FIGURE 2

Analysis of integrin-mediated adhesion in pyk2−/− PMNs. A, PMNs from WT or pyk2−/− mice were allowed to adhere to pRGD-coated coverslips and fixed at the indicated time points. The fixed cells were stained for actin (red). Images shown are representative of three independent experiments. B, Fluorescently-labelled bone marrow PMNs from WT or pyk2−/−mice were plated on pRGD coated wells and then exposed to a series of washes in a static adhesion assay. The decrease in fluorescence corresponding to the decrease in cell number was measured by fold-change from the baseline value was plotted over the series of washes. C, Following the assay design in B, PMNs from WT or pyk2−/− mice were allowed to adhere to fibrinogen coated wells for 15 minutes. Error bars represent ± SEM of triplicate samples. Data shown are pooled from 3 independent experiments.