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. 2011 Dec 29;6(12):e29690. doi: 10.1371/journal.pone.0029690

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Carcamo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Enrichment of CTPS1, detected by rabbit anti-CTPS1 (green), to RR (arrows) identified by It2006 (red). Nuclei were counterstained with DAPI (blue). (B) IMPDH2 stained by rabbit anti-IMPDH2 (red) localized to RR (arrows) detected by It2006 (green). Bar, 10 µm. (C) Immunoprecipitation (IP) analysis using an extract of [³⁵S]-methionine-labeled K562 cells and rabbit anti-IMPDH2, It2006, and a second human anti-RR serum 609. It2006 recognized a 55 kDa protein band that co-migrated with IMPDH2 immunoprecipitated by rabbit anti-IMPDH2. Serum 609 did not immunoprecipitate the 55 kDa protein. (D) IP-Western blot demonstrated that serum It2006 immunoprecipitated IMPDH2, which was recognized by both mouse monoclonal and rabbit anti-IMPDH2 antibodies. NHS, control normal human serum.