Figure 10. Uninduced mouse embryonic stem cells expressed predominantly cytoplasmic rings that were disassembled during retinoic acid-induced differentiation and reassembled when treated with Acivicin.

(A) Mouse 3T3 cells for comparison shown with both rods (80–90%, arrowhead) and rings (10–20%, arrows). (B) Undifferentiated mouse ESCs shown with ∼90% rings (arrows) and few rods. (C) ESCs treated with RA for 4 days showed no RR. (D) RR were induced in these RA-differentiated cells by treatment with 2 mM Acivicin for 24 h. Cells were stained with human anti-RR serum It2006 (green, A–D) and co-stained with rabbit anti-giantin (red, A, B only). Nuclei counterstained with DAPI (blue). The percentage of ESCs with RR displayed for each condition is shown in the lower right corner with the total number of cells counted indicated in parentheses. Bar, 5 µm.