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. 2011 Dec 29;6(12):e29778. doi: 10.1371/journal.pone.0029778

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Hoopes et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Wild type influenza A/Hawaii/31/2007 (H1N1) virus was passaged in the presence of escalating concentrations of each drug regimen for a total of ≥25 cumulative days in culture, or until the drug concentration reached the 50% of the cytotoxic concentration (TC₅₀) of the drug as a single agent.