Table 3. Up-regulation of P450 genes in adults of permethrin selected offspring of the field populations of Culex quinquefasciatus.
Relative Gene expression ± SEb | |||||
Mosquitoes | Transcript IDa | Gene | Parental strainc | Resistant straind | Ratioe |
HAmCq (17) | CPIJ017199 | CYP325BF1v1 | 0.9±0.1 | 1.8±0.3 | 2.0 |
CPIJ019587 | CYP6Z14 | 1.1±0.3 | 2.3±0.7 | 2.1 | |
CPIJ010536 | CYP9J44 | 0.9±0.06 | 1.9±0.2 | 2.1 | |
CPIJ005959* # | CYP6AA7 | 1.4±0.2 | 3.2±1.0 | 2.3 | |
CPIJ015959 | CYP325BJ1 | 1.9±0.1 | 4.3±0.2 | 2.3 | |
CPIJ015318 | CYP325V5v2 | 0.9±0.02 | 2.2±0.5 | 2.4 | |
CPIJ010548* | CYP9J39 | 1.0±0.1 | 2.5±0.1 | 2.5 | |
CPIJ016284 | CYP4J4 | 0.8±0.05 | 2.0±0.2 | 2.5 | |
CPIJ011127 | CYP4H34 | 0.9±0.08 | 2.4±0.3 | 2.7 | |
CPIJ010203 | CYP9AM1 | 0.9±0.1 | 2.7±0.6 | 3.0 | |
CPIJ001758 | CYP4H38 | 0.7±0.4 | 2.2±0.7 | 3.1 | |
CPIJ009085 | CYP6AG13 | 0.6±0.2 | 1.9±0.2 | 3.2 | |
CPIJ012640 | CYP6CP1 | 0.9±0.08 | 3.3±1.1 | 3.7 | |
CPIJ018943* # | CYP4C52v1 | 0.8±0.02 | 3.6±2.0 | 4.5 | |
CPIJ006721 | CYP4H37v1 | 0.6±0.05 | 2.9±0.8 | 4.8 | |
CPIJ003389 | CYP6BY7 | 0.5±0.2 | 2.7±0.08 | 5.4 | |
CPIJ010230 | CYP12F10 | 1.8±0.01 | 10±2.0 | 5.6 | |
MAmCq (15) | CPIJ005957 | CYP6AA9 | 0.9±0.09 | 1.9±0.4 | 2.1 |
CPIJ015953 | CYP325BF1v2 | 2.4±0.8 | 5.3±1.8 | 2.2 | |
CPIJ007092 | CYP325Y7 | 0.9±0.01 | 2.1±0.4 | 2.3 | |
CPIJ015961 | CYP325BE1 | 1.1±0.2 | 2.5±1.3 | 2.3 | |
CPIJ010548* # | CYP9J39 | 0.9±0.04 | 2.2±0.2 | 2.4 | |
CPIJ007091 | CYP325Y6 | 1.3±0.3 | 3.2±1.7 | 2.5 | |
CPIJ007090 | CYP325Y5 | 0.8±0.03 | 2.0±1.1 | 2.5 | |
CPIJ005959* # | CYP6AA7 | 1.3±0.5 | 3.4±1.0 | 2.6 | |
CPIJ015954 | CYP325N3v1 | 0.7±0.2 | 1.9±0.5 | 2.7 | |
CPIJ006952 | CYP325BG3 | 1.7±0.07 | 5.9±0.8 | 3.5 | |
CPIJ010272 | CYP325BK2 | 0.9±0.07 | 4.1±1.0 | 4.6 | |
CPIJ014730 | CYP325AA2 | 0.3±0.2 | 1.9±0.6 | 6.3 | |
CPIJ017021 | CYP325K3v1 | 3.0±0.09 | 20±1.6 | 6.7 | |
CPIJ005685 | CYP325BB2 | 0.9±0.1 | 6.1±2.0 | 6.8 | |
CPIJ018943* # | CYP4C52v1 | 0.2±0.02 | 2.8±0.7 | 14 |
The transcript ID number from the vectorbase of the Cx. quinquefasciatus genome sequence (http://cquinquefasciatus.vectorbase.org/).
The relative level of gene expression represents the ratio of the gene expression in each resistant strain compared with that in the susceptible S-Lab strain. The relative level of gene expression for S-Lab is 1.
Parental strain for HAmCq population is HAmCqG0 and for MAmCq population is MAmCqG0.
Permethrin selected strain for HAmCq population is HAmCqG8 and for MAmCq population is MAmCqG6.
The ratio of the relative gene expression in each permethrin selected strain compared its parental strain.
*The genes that are up regulated in both adult of HAmCqG8 and MAmCqG6
The genes that are up regulated in both larvae and adults of each of HAmCqG8 and MAmCqG6, or both.