Skip to main content
. 2011 Dec 29;6(12):e29758. doi: 10.1371/journal.pone.0029758

Figure 4. qPCR validation of differential expression of Twist1 candidate target genes in siRNA-transfected MC3T3-E1 cells and in ECC and remodeling valves in vivo.

Figure 4

A. qPCR was performed to determine the level of expression of candidate Twist1 target genes in MC3T3-E1 cells transfected with siTwist1 versus siScr control. Fold change in expression relative to the level in siScr control cells is shown. B. qPCR was performed to determine the level of expression of Twist1 candidate target genes in mouse E17.5 AV valves versus E12.5 ECCs. Fold change in expression relative to the level in E12.5 ECCs is shown. qPCR determination of the expression of the known Twist1 target gene Col2a1 was included as a positive control. Statistical significance was determined by Student's t-Test, p = ≤0.05 indicated by *. All experiments were performed in biological and technical triplicates. Histograms are a compilation of n = 3 experiments.