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. 2011 Dec 29;6(12):e29456. doi: 10.1371/journal.pone.0029456

Figure 3. Expression of the PVRN1:GFP and PVRN1EX1-2::GFP constructs in control versus cold treated seedlings.

Figure 3

(A) GFP transcript levels in transgenic seedlings carrying the PVRN1:GFP construct. Expression was assayed by quantitative reverse transcriptase PCR in control seedlings, germinated in darkness at normal glasshouse temperatures (20 degrees for 5 days), compared to seedlings germinated and grown in darkness to an identical stage of development at low temperatures (4 degrees for 28 days). Expression levels were assayed in three independent transgenic lines. (B) Expression of the PVRN1EX1-2::GFP, assayed as outlined above, in three independent transgenic lines. Expression is shown relative to ACTIN. Error bars show standard error for a minimum of 3 biological replicates. * indicates P<0.05.