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. 2011 Dec 29;6(12):e29269. doi: 10.1371/journal.pone.0029269

Figure 5. Deletion constructs of the large cytoplasmic loop of the Na+,K+-ATPase α-subunit and GST pull down of PP2A with GST constructs.

Figure 5

A. HA tagged PP2A C-subunit was expressed in COS cells and cell lysates were incubated with GST fusion proteins. PP2A C-subunit was detected by Western blot with anti-HA antibody (upper panel) and GST fusion proteins were detected by CBB staining. Not only N-terminal segments but also the C-terminal half of the large cytoplasmic loop binds to PP2A C-subunit. Typical results from one of four experiments are shown. B. Flag tagged PP2A A-subunit was expressed in COS cells and cell lysates were incubated with GST fusion proteins. PP2A A-subunit was detected by Western blot with anti-flag antibody (upper panel) and GST fusion proteins were detected by CBB staining. The PP2A A-subunit co-precipitated with the A-domain of the Na+,K+-ATPase α-subunit. Typical results from one of three experiments are shown.