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. 2011 Dec 29;6(12):e29269. doi: 10.1371/journal.pone.0029269

Figure 8. Immunolocalization of the Na+,K+-ATPase and arrestin in the presence of PP2A in COS cells.

Figure 8

COS cells were transfected with H85N plus Na+,K+-ATPase β-subunit (A), with H85N, Na+,K+-ATPase β-subunit and flag tagged arrestin (B-D), with H85N, Na+,K+-ATPase β-subunit and HA tagged PP2A C-subunit (E-G), or with H85N plus Na+,K+-ATPase β-subunit and flag tagged arrestin plus HA tagged PP2A C-subunit (H-M). Cells were stained with HK9 (A, B, E, H and K), anti-flag for arrestin (C and I), and anti-HA for PP2A C-subunit (F and L) antibodies. Overlay patterns are shown in D, G, J and M. (×200 magnification) A large fraction of the H85N was found in intracellular compartments when cells expressed arrestin in the absence of PP2A C-subunit. This effect was not observed when arrestin was expressed together with the PP2A C-subunit. Typical results from one of three experiments are shown.