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. 2011 Dec 29;7(12):e1002429. doi: 10.1371/journal.pgen.1002429

Figure 4. InR transgene expressed under heterologous promoter is insensitive to KSR depletion.

Figure 4

(A) Immunoblots to visualize the level of Flag-tagged InR protein expressed under control of the pMT promoter in transfected S2 cells. Cells were treated with dsRNA to deplete PI3K, KSR or GFP as a control and after 5 days stimulated with insulin. Insulin activity was visualized using antibody specific to the phosphorylated form of InR (upper panel). Total Flag-InR was visualized with anti-Flag. Anti-Kinesin was used as loading control. Samples were run on the same gel, but intervening lanes have been removed as indicated. (B) Adult eyes expressing GMR-Gal4 and UAS-FOXO, UAS-InR and/or a UAS-ksrRNAi transgene to deplete KSR. (C) Quantification of the total eye area measured in pixels from digital images using ImageJ. Error bars indicate standard deviation from measurement of at least 5 eyes for each genotype. Student's t-test: (***) p<0.001. (N.S.) indicates that there was no significant effect of KSR depletion in the UAS-FOXO+UAS-InR eyes.