Table 2. Primers used for cloning into pCIG2 vector.
Primer namea | Sequence (5′ to 3′)b |
UL133-Fwd | ccggaattcgctagcaccATGGGTTGCGACGTGCACGATCCTTCG |
UL135-Fwd | cgcggatccgctagcaccATGTCCGTACACCGGCCCTTCCCGACA |
UL136-Fwd | ccggaattcgctagcaccATGTCAGTCAAGGGCGTGGAGATGCCA |
UL138-Fwd | ggggGCTAGCACCATGGACGATCTGCCGCTGAACGTCG |
C-Myc-Rev | cgcggatccTCACAGATCCTCTTCTGAGATGAGTTTTTGTTC |
UL1333xFLAG-Rev | ggggGATATCTTACTTGTCGTCGTCGTCCTTGTAGTCGAATTCCTTGTC |
GTCGTCGTCCTTGTAGTCTGCCCCTTTATCATCATCATCTTTATAATC | |
ACCGCCACCGCCCGTTCCGGTCTGATGC | |
UL135HA-Rev | gggggatatcTCACGCGTAATCTGGAACATCGTATGGGTAACCGCCAC |
CGCCGGTCATCTGCATTGACTCGGCGTCC | |
UL136myc-Rev | gggggatatcTTACAGATCCTCTTCTGAGATGAGTTTTTGTTCACCGCC |
ACCGCCCGTAGCGGGAGATACGGCGTTCTCC | |
UL138EE-Rev | gggggatatcTCACTCCATGGGCATGTACTCACCGCCACCGCCCGTGTA |
TTCTTGATGATAATGTACC | |
Bleo-Fwd | ggggaccggtaccATGGCCAAGTTGACCAGTGCCGTTCCG |
Bleo-Rev | gggggcggccgcTCAGTCCTGCTCCTCGGCCACG |
Neo-Fwd | ggggaccggtaccATGGGATCGGCCATTGAACAAGATGGATTGC |
Neo-Rev | gggggcggccgcTCAGAAGAACTCGTCAAGAAGGCGATAGAAGG |
Puro-Fwd | ggggaccggtaccATGACCGAGTACAAGCCCACGGTGCG |
Puro-Rev | gggggcggccgcTCAGGCACCGGGCTTGCGGGTC |
Hygro-Fwd | ggggaccggtaccATGGATAGATCCGGAAAGCCTGAACTCACC |
Hygro-Rev | gggggcggccgcCTATTCCTTTGCCCTCGGACGAGTGC |
Fwd: Forward; Rev: Reverse
Enzyme sites are in lower case