Fig. 1.

YFP expression marks Rb-deficient cellular mosaic in Chx10-Cre;Rb^lox/lox;Rosa-YFP mouse retinas. (A) Confocal image of YFP expression in a column of cells in Chx10-Cre;Rb^lox/lox;Rosa-YFP P18 mouse retinas. (B) Overlay of YFP expression (green) with nuclear staining with DAPI (blue) to highlight the retinal laminar structure. Individual cell types are indicated, including a cell in the ganglion cell layer (arrowhead). (C) 3D z-series through another column of cells that underwent Cre-mediated recombination of Rb^Lox and the Rosa-EYFP reporter gene. (D and E) Confocal image of a horizontal section through the retina shown in A and B at the apical portion level of the INL, highlighting the mosaic pattern of the Chx10-Cre transgene activity. Arrowhead indicates mosaic patch of Rb-deficient cells, and the open arrowhead indicates a patch of normal retina. Am, amacrine cell; GCL, ganglion cell layer; HC, horizontal cell; INL, inner nuclear layer; IPL, inner plexiform layer; is, inner segments of photoreceptors; Mü, Müller glia; ONL, outer nuclear layer; OPL, outer plexiform layer. (Scale bars: 10 μm.)