Fig. 1.
Time course experiment: the addition of labeled sulfide (product) to highly enriched, detritus-free consortia from Isis MV that perform anaerobic oxidation of methane. (A) Developing sulfide concentrations in the presence (•) and absence (○) of methane. (B) Development of 35S-activity in the sulfate pool during incubation with (▲) and without (△) methane as electron donor. The total 35S-activity (◆, sulfate + sulfide) is also indicated. The shown radiolabel was determined after incubation at the same time to eliminate the effect of decay. (C) Double logarithmic plot according to Eq. 16 to calculate the reverse rate; dotted line marks the 95% confidence interval.