Fig. 3.

ISGF3 binding decreases, whereas GAF binding increases in IKKε knockout MEFs. (A) EMSA with extracts derived from Ikbke^+/+ and Ikbke^−/− MEFs stimulated with IFNβ for the time points indicated. EMSA probes include IKKε-independent ISRE, IKKε-dependent ISRE, or IRF GAS element. (B) EMSA with extracts derived from Ikbke^+/+ and Ikbke^−/− MEFs stimulated with IFNβ or IFNγ for time points indicated and analyzed as in A. Unbound probe was used as loading control. Experiments were independently repeated at least three times.