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. 2011 Dec 29;7(12):e1002450. doi: 10.1371/journal.ppat.1002450

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Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Yeast transformants expressing bait (pGBKT7) and prey (pGADT7) constructs were assayed for growth on SD-Leu-Trp-His (SD-His) plates and β-galactosidase (LacZ) activities with positive and negative control. (B) co-IP assay for the interaction of MoRgs2 with MoMagB. Western blot analysis with total proteins (Total) isolated from transformants co-expressing the MoRGS2-GFP and MoMAGB-3xFLAG constructs and proteins eluted from the anti-FLAG M2 beads (Elution). The presence of MoRgs2 and MoMagB was detected with an anti-GFP and an anti-FLAG antibody, respectively. Total proteins isolated from the wild-type strain (70-15 fractions) and detection with an anti-actin antibody was included as the controls.