Table 1. Real-time RT-PCR quantification of MoRGS gene expression in M.oryzae.
| Nomalized gene level relative to Actina | ||||||||
| RNA source (Wild-type) | MoRGS1 | MoRGS2 | MoRGS3 | MoRGS4 | MoRGS5 | MoRGS6 | MoRGS7 | MoRGS8 |
| Mycelium | 1.00(0.6–1.67) | 1.00(0.95–1.05) | 1.00(0.97–1.03) | 1.00(0.82–1.22) | 1.00(0.77–1.30) | 1.00 (0.80–1.25) | 1.00 (0.96–1.04) | 1.00 (0.88–1.14)b |
| Conidium | 58.22(53.28–63.61) | 48.84(44.45–53.66) | 44.22(42.12–46.43) | 4.22(3.44–5.17) | 7.75(6.72–8.92) | 82.6(80.61–91.63) | 88.49(74.75–85.31) | 5.95(5.03–7.04) |
| Appressorium | 59.58 (47.96–74.01) | 3.45 (2.86–4.17) | 19.65 (17.94–21.53) | 31.41(30.63–32.22) | 11.55 (8.66–15.41) | 87.43 (73.42–104.11) | - | - |
| Infection stage | 12.5 (9.59–16.29) | 3.29 (3.02–3.58) | 10.78 (9.74–11.93) | 1.94 (0.84–4.47) | 2.40 (1.00–5.75) | 4.05 (2.84–5.76) | 17.76(16.73–18.85) | 2.01(1.90–2.12) |
a
Relative quantity of MoRGS genes at different developmental stages of the wild-type strain Guy11.
b
The mean and range of three replicates.