Table 1. DNA methylation analyses of HygR colonies obtained in cotransformations of N644 with hH3 alleles.
| Substitution | Number of transformations | Fraction yielding resistance to hygromycin1 | Fraction hypomethylated2 |
| R2L | 6 | 3/6 | 0/33 |
| K4L | 6 | 0/6 | - |
| T6A | 4 | 1/4 | 0/14 |
| A7M | 4 | 0/4 | - |
| R8A | 5 | 3/5 | 1/35 |
| K9L | 8 | 3/8 | 3/3 |
| K9R | 8 | 3/8 | 3/3 |
| S10A | 8 | 5/8 | 5/5 |
| T11A | 6 | 5/6 | 5/5 |
| G12P | 6 | 3/6 | 1/3 |
| +G13 | 7 | 5/7 | 3/5 |
| G13M | 5 | 4/5 | 2/4 |
| K14Q | 8 | 7/8 | 6/7 |
| K14R | 8 | 7/8 | 7/7 |
| A15M | 6 | 1/6 | 0/16 |
| P16A | 4 | 0/4 | - |
| R17L | 4 | 1/4 | 0/16 |
| K18Q | 6 | 1/6 | 0/16 |
| K18R | 6 | 2/6 | 1/27 |
| K23Q | 6 | 0/6 | - |
| K23R | 6 | 1/6 | 0/16 |
| K27L | 6 | 0/6 | - |
| S28A | 6 | 0/6 | - |
| K36A | 6 | 0/6 | - |
1
Fraction transformations that gave rise to HygR colonies.
2
Fraction transformations that showed loss of DNA methylation in Southern blotting.
3
One transformant out of 12 tested exhibited hypomethylation.
4
Of the two HygR transformants obtained, both were methylated.
5
Half of the transformants tested (28) demonstrated loss of DNA methylation.
6
All HygR transformants tested were methylated.
7
Two HygR transformants out of 11 tested were hypomethylated.