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. 2011 Dec 29;7(12):e1002434. doi: 10.1371/journal.pgen.1002434

Figure 2. ocp1 is a mutant allele of NRPD2.

Figure 2

(A) OCP1 corresponds to At2g27040 encoding NRPD2. The G nucleotide residue deleted in the ocp1 allele is indicated in red bold uppercase letters in the wild-type sequence. Deduced amino acid sequences are indicated below each nucleotide triplet, and the first amino acid change (S to T) where the frameshift of the OCP1 protein starts is shown in blue. (B) NRPD2 expression level by RT-PCR in mRNAs derived from Col-0, nrpd2-2 and ocp1 plants. The eEF1a house-keeping gene was used as a control. (C–D) nrpd2 plants show enhanced susceptibility to fungal pathogens. Lesion size was measured in Col-0, ocp1 and nrpd2-2 plants after inoculation with B. cinerea (C) or P. cucumerina (D). Data points represent average lesion size ± SE (n≥30 lesions). ANOVA detected significant differences at the P<0.05 level. (E–F) RT-qPCR determination of PDF1.2a (E) and PR-1 (F) transcript levels following inoculation with P. cucumerina. Data represent the mean ± SD; n = 3 biological replicates.