Figure 3. ocp1 plants show hypomethylation in RdDM target DNA sequences.
Genomic DNA isolated from Col-0, ocp1 and ago4-2/ocp11 plants (A) and nrpd2, nrpd1 and nrpe1 (B) was digested (+) or not (−) with HaeIII and amplified by PCR for SUPERMAN promoter (A), the ribosomal 5S genes and the retrotransposon AtSN1 (B). ABI5 contains no target sequences for HaeIII and was used as a control.