Figure 9. Effects on H2 production caused by amiRNA-mediated knock-down of MOC1.
A: MOC1 mRNA expression in the MOC1 knock-down strain (MOC1-RNAi; grey bar) and the WT parental strain (parental; black bar) as determined by RT-Q-PCR. MOC1 expression in the knock-down strain is given relative to the expression in the parental strain (set to 100%). Error bars indicate the standard deviation from three biological experiments. B: Representative immunoblot experiment (αMOC1) using an antiserum raised against MOC1 and the MOC1 knock-down strain (MOC1-RNAi) as well as its parental strain (parental). A Coomassie brilliant blue stain serves a loading control (CBB). C: Representative H2 measurement with the MOC1 knock-down (grey curves) and parental strain (black curves). Total H2 production (right y-axis; total H2; continuous lines) and the change of the photosynthetic quantum yield (left y-axis; ΦPSII; dotted lines) were recorded. The duration of the sulphur deplete condition is given on the x-axis. Indicated standard deviations (error bars) are derived from three technical replicates per strain. D: Result from four independent H2 experiments with the MOC1 knock-down strain (MOC1-RNAi; grey bar) and parental strain (parental; black bar). The H2 capacity (total volume of H2 produced) is calculated relative to the parental strain which was set to 100%. Error bars represent the standard deviation from four biological experiments with three technical replicates per experiment.