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AMPK dose-dependently shortened the time to maximal activation of KATP channels by metabolic inhibition. Cells (n=5–9) were incubated with various concentrations of AICAR before metabolic inhibition (as in the previous figure). The time to maximal activation of KATP channels is plotted as a function of the AMPK concentration. The line was drawn by fitting data points to a Boltzman distribution.
